Gene | Spag4 |
Chromosome | 2 |
Deletion Start | 156,065,361 |
Deletion End | 156,069,475 |
Deletion Size | 4,115 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 156,066,215 | 156,066,234 | CGAGGGTGTCCAAGAGCTTC |
TUP | 156,066,235 | 156,066,256 | CTGAGTACGAGCCAGGCCAGCC |
TUR | 156,066,310 | 156,066,328 | GCATCTGGGCTGGTTTCTC |
TDF | 156,069,034 | 156,069,056 | TACCATGAGAACCTAAGCCTGAG |
TDP | 156,069,059 | 156,069,086 | CGCATACTGTGGAATAGTTGGCTTTCCC |
TDR | 156,069,097 | 156,069,117 | GAAGAACAGTGAGGCCCAGAG |
PCR Primers
Primer | Sequence |
SU | TGGCTCCCAGTGACGTCA |
SD | TCACTCGTACTTGACTGAGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 114 bp | + | + | - |
TDF | TDR | 84 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
12063A-A5 | VGB6 | C57BL/6NTac | |
12063A-B3 | VGB6 | C57BL/6NTac | |
12063A-D9 | VGB6 | C57BL/6NTac | |
12063A-G12 | VGB6 | C57BL/6NTac | |