| Gene | Gimap9 |
| Chromosome | 6 |
| Deletion Start | 48,677,484 |
| Deletion End | 48,678,439 |
| Deletion Size | 956 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,677,523 | 48,677,545 | GGGAAGACTGGAAATGGGAAGAG |
| TUP | 48,677,546 | 48,677,569 | TGCTACAGCCAACACCATCCTCGG |
| TUR | 48,677,591 | 48,677,612 | GGTAACAGCATTGGCACAAATC |
| TDF | 48,677,986 | 48,678,009 | ACAAAGCAGCTCTGGATGAGCAAG |
| TDP | 48,678,014 | 48,678,041 | TCAAGTGCAGCAGCTGATAGAACTGACA |
| TDR | 48,678,044 | 48,678,064 | CCATTTTGGGCCACCATTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGAATGCCTCTCCTAGGTCG |
| SD | ATCGCTCTGAAGGTCAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12071A-H8 | VGB6 | C57BL/6NTac | |
| 12071A-B7 | VGB6 | C57BL/6NTac | |
| 12071A-H9 | VGB6 | C57BL/6NTac | |
| 12071A-A5 | VGB6 | C57BL/6NTac | |
| 12071A-F11 | VGB6 | C57BL/6NTac | |
