| Gene | Aifm3 |
| Chromosome | 16 |
| Deletion Start | 17,492,076 |
| Deletion End | 17,507,243 |
| Deletion Size | 15,168 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,492,466 | 17,492,484 | TGCCCTCTTAGGGTGACTG |
| TUP | 17,492,485 | 17,492,512 | ACAGGGTATCATGGACAGGTCCCTACTC |
| TUR | 17,492,547 | 17,492,569 | ACAGGAGATGAGGCCCAGAGAAG |
| TDF | 17,507,090 | 17,507,108 | TCTTCTGCCCTGACCCATC |
| TDP | 17,507,110 | 17,507,134 | TCCTCTCATTCCTGCAGAACCGGTG |
| TDR | 17,507,168 | 17,507,188 | CCCAAGTCCACCACAGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGCACAGAGGGTCTCCAAC |
| SD | AAGTGTCGGCCCAATTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12073A-H9 | VGB6 | C57BL/6NTac | |
| 12073A-H3 | VGB6 | C57BL/6NTac | |
| 12073A-G12 | VGB6 | C57BL/6NTac | |
| 12073A-B10 | VGB6 | C57BL/6NTac | |
