| Gene | Gdnf |
| Chromosome | 15 |
| Deletion Start | 7,811,057 |
| Deletion End | 7,834,867 |
| Deletion Size | 23,811 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,811,906 | 7,811,929 | GGGAGTAGGAAGGCACTTTAACAG |
| TUP | 7,811,932 | 7,811,954 | TCCTCCCTGCTTGCAATCTTGCC |
| TUR | 7,812,009 | 7,812,027 | TGGCGAACTCAGGGCTAAG |
| TDF | 7,834,259 | 7,834,279 | GGGTCGGATTCTAGCGAGTTC |
| TDP | 7,834,280 | 7,834,307 | CCGTGACTTAGAAGACTGCCCGAAATGA |
| TDR | 7,834,343 | 7,834,365 | AATCTTCAGGCATATTGGCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TTTGACGTGGTGTCTCGTTC |
| SD | GAATCGTCACATTTCCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12074A-C7 | VGB6 | C57BL/6NTac | |
| 12074B-F4 | VGB6 | C57BL/6NTac | |
| 12074B-D12 | VGB6 | C57BL/6NTac | |
