| Gene | Ogfod2 |
| Chromosome | 5 |
| Deletion Start | 124,112,341 |
| Deletion End | 124,115,040 |
| Deletion Size | 2,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,113,066 | 124,113,087 | GGGCAGTGTCAGGTTTAAGAAG |
| TUP | 124,113,089 | 124,113,111 | ATAGGGAGACTTGCCCTCCAGCC |
| TUR | 124,113,119 | 124,113,141 | CCCAGGAAGACCAAAGTGAAGTG |
| TDF | 124,114,844 | 124,114,862 | CTGCGGGCATCTGCTGTTC |
| TDP | 124,114,865 | 124,114,889 | AATCGCCTCTGTCCCATGTGCTGCC |
| TDR | 124,114,910 | 124,114,929 | CCGTCACCGAAACCCTCATC |
PCR Primers
| Primer | Sequence |
| SU | ACTGTCTACGTCCTGTGAC |
| SD | TGGACTGTCACCACAACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12075A-A10 | VGB6 | C57BL/6NTac | |
| 12075A-B2 | VGB6 | C57BL/6NTac | |
| 12075A-B9 | VGB6 | C57BL/6NTac | |
| 12075A-C4 | VGB6 | C57BL/6NTac | |
| 12075A-G9 | VGB6 | C57BL/6NTac | |
| 12075A-H9 | VGB6 | C57BL/6NTac | |
