| Gene | Tmem186 |
| Chromosome | 16 |
| Deletion Start | 8,637,485 |
| Deletion End | 8,635,706 |
| Deletion Size | 1,780 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,637,206 | 8,637,228 | TGAAGAAGGATGCACAGCTAGTG |
| TUP | 8,637,177 | 8,637,202 | TCTTGACTCCAAAGCCCAGAATCTGC |
| TUR | 8,637,156 | 8,637,175 | GCCTGTGGTTCTCTGGAGTG |
| TDF | 8,636,223 | 8,636,246 | CACCGAGACCGAAACATTCCATAC |
| TDP | 8,636,198 | 8,636,219 | CTACCGGTTCCGAGCCATCCGA |
| TDR | 8,636,167 | 8,636,185 | CCAACTTCAGCCGCGAGAG |
PCR Primers
| Primer | Sequence |
| SU | CATCCATGTCGAAGAGACAG |
| SD | TTTCCATCTCACAATGCAATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12077A-D6 | VGB6 | C57BL/6NTac | |
| 12077A-G3 | VGB6 | C57BL/6NTac | |
| 12077A-B7 | VGB6 | C57BL/6NTac | |
| 12077A-D2 | VGB6 | C57BL/6NTac | |
