| Gene | Acadvl |
| Chromosome | 11 |
| Deletion Start | 70,015,321 |
| Deletion End | 70,010,198 |
| Deletion Size | 5,124 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,014,956 | 70,014,976 | CAGGGTGCCCTAGTGAGAAAG |
| TUP | 70,014,928 | 70,014,952 | TGGAACATGCACCAAACGCACTTCC |
| TUR | 70,014,872 | 70,014,893 | CAGAGCAGTTAGGCTGGAGATG |
| TDF | 70,010,592 | 70,010,613 | TGTTTCCTCACAGAGCCTCAAG |
| TDP | 70,010,569 | 70,010,590 | TCCCTGAGTGAGGGCTACCCGA |
| TDR | 70,010,521 | 70,010,542 | TCACCTCAATGCACCAGCTATC |
PCR Primers
| Primer | Sequence |
| SU | CCTCCCTCCAGTCTACCTCC |
| SD | CAGAATATGACCGCCACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12078A-A9 | VGB6 | C57BL/6NTac | |
| 12078A-C8 | VGB6 | C57BL/6NTac | |
| 12078A-E8 | VGB6 | C57BL/6NTac | |
| 12078A-G11 | VGB6 | C57BL/6NTac | |
| 12078A-G12 | VGB6 | C57BL/6NTac | |
