| Gene | Nphs2 |
| Chromosome | 1 |
| Deletion Start | 156,302,811 |
| Deletion End | 156,318,249 |
| Deletion Size | 15,439 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 156,311,362 | 156,311,384 | CAACAACCTTGTGCCTCCTCTTC |
| TUP | 156,311,387 | 156,311,412 | TCGTCCTTGCCAATCAGTCTTAAGCC |
| TUR | 156,311,441 | 156,311,459 | GGGCTGGTCGGTGAAAGTC |
| TDF | 156,325,937 | 156,325,955 | CGCTGTCCAGCTTCGATAC |
| TDP | 156,325,957 | 156,325,980 | TGCACACTCTTCAGTCGCTGTCCA |
| TDR | 156,326,048 | 156,326,067 | TGCTCCCTTGTGCTCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTAGCCACCTAAGCAGAGCC |
| SD | AGATGCCTGTGTCCTAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 131 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12079A-E3 | VGB6 | C57BL/6NTac | |
| 12079B-G8 | VGB6 | C57BL/6NTac | |
