| Gene | C1galt1c1 |
| Chromosome | X |
| Deletion Start | 38,632,114 |
| Deletion End | 38,631,068 |
| Deletion Size | 1,047 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,632,020 | 38,632,042 | GGCCACATTAGGATTGGAAACAG |
| TUP | 38,631,994 | 38,632,017 | TGCACCACCATGAGCATCACCATC |
| TUR | 38,631,938 | 38,631,957 | CTCCATGCGTTCAGCCTCTG |
| TDF | 38,631,294 | 38,631,316 | TGTTGCTCTGATATGGCTGTTAC |
| TDP | 38,631,259 | 38,631,287 | TGGACTGACTCCTAATCAGATGCACGTGA |
| TDR | 38,631,226 | 38,631,247 | CAAATGCCCTAAGCCGGTACAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCAATCCTTGAATGAGTTTC |
| SD | ACTTTCTCAAGCATGGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12081B-B1 | VGB6 | C57BL/6NTac | |
| 12081B-F6 | VGB6 | C57BL/6NTac | |
| 12081B-H11 | VGB6 | C57BL/6NTac | |
| 12081B-H5 | VGB6 | C57BL/6NTac | |
