| Gene | Lamtor2 |
| Chromosome | 3 |
| Deletion Start | 88,552,860 |
| Deletion End | 88,549,945 |
| Deletion Size | 2,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,552,333 | 88,552,356 | TGGTATGGAGTAAAGTAGGCTGAC |
| TUP | 88,552,298 | 88,552,325 | TTGCAATGATTATCCTGCTTCTGCCACC |
| TUR | 88,552,257 | 88,552,274 | TGGCAAGGTGCGATGGTG |
| TDF | 88,550,008 | 88,550,027 | ACTGTGCTCCTGCCTCCTTG |
| TDP | 88,549,981 | 88,550,002 | CCAGGCCCTGGTGCAGTACCTG |
| TDR | 88,549,947 | 88,549,971 | GTTATGATGCTGCTACTTGGGTGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTGCGATTGACCAGTAGGC |
| SD | AATTCTGGGAGGATGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12082A-B12 | VGB6 | C57BL/6NTac | |
| 12082A-H4 | VGB6 | C57BL/6NTac | |
