| Gene | 8030474K03Rik |
| Chromosome | X |
| Deletion Start | 101,794,757 |
| Deletion End | 101,798,126 |
| Deletion Size | 3,370 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,795,572 | 101,795,592 | TGTACTGTCCAGTGCTACCTG |
| TUP | 101,795,596 | 101,795,619 | AGTCAGCTCAGGCATGGAAAGGCA |
| TUR | 101,795,630 | 101,795,650 | AGCCAAAGAGCTTTCCTGTTC |
| TDF | 101,797,155 | 101,797,173 | CCTGCAAACTGACCTCCTC |
| TDP | 101,797,174 | 101,797,202 | TCAGATGCTGGATTAGGTTGTACCAAGCA |
| TDR | 101,797,211 | 101,797,231 | GCTTTGAGCCTTGCTTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | AAGTCTCGCGAGACTTCGC |
| SD | ATAGGACACTCACTTCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12083A-C12 | VGB6 | C57BL/6NTac | |
| 12083A-D1 | VGB6 | C57BL/6NTac | |
| 12083A-G3 | VGB6 | C57BL/6NTac | |
| 12083A-H2 | VGB6 | C57BL/6NTac | |
