| Gene | Gm1673 |
| Chromosome | 5 |
| Deletion Start | 33,983,494 |
| Deletion End | 33,985,062 |
| Deletion Size | 1,569 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,983,923 | 33,983,942 | GGAGGCGTCTTTGTTTCCTG |
| TUP | 33,983,943 | 33,983,967 | AGCGGTCACTTCTTGGGTGGGAACT |
| TUR | 33,984,001 | 33,984,020 | GTGGACAGACCAAGCCACTG |
| TDF | 33,984,628 | 33,984,647 | GGGCCAGAGTTCATGCTTTG |
| TDP | 33,984,651 | 33,984,679 | ATCCATGTGAGTTGAAAGATGGCTGGAGT |
| TDR | 33,984,697 | 33,984,716 | GGTCCCTCCTCACACTCATC |
PCR Primers
| Primer | Sequence |
| SU | GAACTGAACGGTCACTGTG |
| SD | GCCCTAGGTAAGGGTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12084A-C11 | VGB6 | C57BL/6NTac | |
| 12084A-E5 | VGB6 | C57BL/6NTac | |
| 12084A-F3 | VGB6 | C57BL/6NTac | |
| 12084A-F6 | VGB6 | C57BL/6NTac | |
