| Gene | Pole3 |
| Chromosome | 4 |
| Deletion Start | 62,524,993 |
| Deletion End | 62,523,938 |
| Deletion Size | 1,056 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,524,813 | 62,524,832 | CCGGACGGTGTCAACATCTC |
| TUP | 62,524,789 | 62,524,811 | AAGGAGGCCCGAAGCGCCATCTC |
| TUR | 62,524,744 | 62,524,764 | GCTCACCAGGATGTGGCATAC |
| TDF | 62,524,443 | 62,524,461 | TGAAGGGCGTTGCTCTTTC |
| TDP | 62,524,412 | 62,524,440 | CCAGTGCCAATAACTTCGCAATGAAAGGA |
| TDR | 62,524,378 | 62,524,397 | ACAGCACGTCACTGGCATTG |
PCR Primers
| Primer | Sequence |
| SU | GAGAAACAAGCCTGGAGTGG |
| SD | CCCTGAGCCTTTAGTGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12086A-C4 | VGB6 | C57BL/6NTac | |
| 12086A-H7 | VGB6 | C57BL/6NTac | |
| 12086A-F4 | VGB6 | C57BL/6NTac | |
