| Gene | Rps9 |
| Chromosome | 7 |
| Deletion Start | 3,704,355 |
| Deletion End | 3,706,837 |
| Deletion Size | 2,483 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,704,873 | 3,704,894 | GGTGTAGATTGGCCTGGAACTC |
| TUP | 3,704,898 | 3,704,927 | TGTAAGTCAGTCTTGCCATAAACTGCCAGA |
| TUR | 3,704,934 | 3,704,954 | GTGACACATACCCAGCACTTC |
| TDF | 3,706,114 | 3,706,133 | AAGGGTCACTGCGGCTCAAG |
| TDP | 3,706,141 | 3,706,164 | CCTTGTGAAGGTGTCTGCCAGGCA |
| TDR | 3,706,179 | 3,706,198 | GCACCAAGAGCTGTTACCTG |
PCR Primers
| Primer | Sequence |
| SU | CGTCTCTATGGCTTTCCTGC |
| SD | TCTCGGCAAGGGAAATAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12090A-C5 | VGB6 | C57BL/6NTac | |
| 12090A-H12 | VGB6 | C57BL/6NTac | |
