| Gene | Fscn2 |
| Chromosome | 11 |
| Deletion Start | 120,361,712 |
| Deletion End | 120,368,152 |
| Deletion Size | 6,441 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,362,469 | 120,362,488 | TGGAGCAAAGTCACCCACAG |
| TUP | 120,362,490 | 120,362,510 | TGGTGCTGGTAGCTGCCAACC |
| TUR | 120,362,518 | 120,362,539 | CCTACCTTGCCTCACAGAGATG |
| TDF | 120,367,909 | 120,367,928 | GGAGGTGGGTTCTGGTACAC |
| TDP | 120,367,932 | 120,367,953 | CAGCCATGGAAGCGTGTGCAGC |
| TDR | 120,367,980 | 120,368,000 | CCACGCTCTCGGAACTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCAAGTTGGCTACTGCTTC |
| SD | GTCAGCCACCACTTTCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12091A-A7 | VGB6 | C57BL/6NTac | |
| 12091A-C3 | VGB6 | C57BL/6NTac | |
| 12091A-F8 | VGB6 | C57BL/6NTac | |
