| Gene | Ipp |
| Chromosome | 4 |
| Deletion Start | 116,510,575 |
| Deletion End | 116,538,024 |
| Deletion Size | 27,450 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,510,592 | 116,510,613 | CAAGGCTGCTGACAATTCATTC |
| TUP | 116,510,614 | 116,510,638 | TCCTCTGACAAACATGCCCAGCTCA |
| TUR | 116,510,707 | 116,510,729 | AGCCGATGGACTTGGAAGGTTTC |
| TDF | 116,537,850 | 116,537,869 | TGCTGTGACAGTCAATGGTC |
| TDP | 116,537,871 | 116,537,894 | TCTGTATGTCTCTGGAGGCCGGTC |
| TDR | 116,537,929 | 116,537,953 | GAGGGTTGTAAACTTCAACTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGGCCGTTCTTCATACC |
| SD | TCCCAGAAGTTTCTAGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12092A-A8 | VGB6 | C57BL/6NTac | |
| 12092A-C11 | VGB6 | C57BL/6NTac | |
| 12092A-F7 | VGB6 | C57BL/6NTac | |
| 12092A-G2 | VGB6 | C57BL/6NTac | |
| 12092A-H3 | VGB6 | C57BL/6NTac | |
| 12092A-E1 | VGB6 | C57BL/6NTac | |
