| Gene | Ccdc116 |
| Chromosome | 16 |
| Deletion Start | 17,144,079 |
| Deletion End | 17,141,198 |
| Deletion Size | 2,882 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,143,668 | 17,143,690 | GCACTTTCACACCATGAACACAG |
| TUP | 17,143,638 | 17,143,663 | CCACTTCTTAGTCTCCCAGCTAGGGC |
| TUR | 17,143,598 | 17,143,617 | TGCCCTGAAGGTTCCCAATG |
| TDF | 17,141,963 | 17,141,983 | AACCAGCCAAGACCCTTCAAC |
| TDP | 17,141,937 | 17,141,961 | TGCCTACCTATTGTCCACTCAGGGA |
| TDR | 17,141,883 | 17,141,901 | TGCAGGGCAGGAAAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGATTCCAGTTCTCTGTGC |
| SD | TTGACAACATCTGGAAACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12097A-H5 | VGB6 | C57BL/6NTac | |
| 12097A-H4 | VGB6 | C57BL/6NTac | |
| 12097A-F1 | VGB6 | C57BL/6NTac | |
| 12097A-B9 | VGB6 | C57BL/6NTac | |
| 12097A-C11 | VGB6 | C57BL/6NTac | |
| 12097A-E5 | VGB6 | C57BL/6NTac | |
