| Gene | Tada3 |
| Chromosome | 6 |
| Deletion Start | 113,376,043 |
| Deletion End | 113,366,958 |
| Deletion Size | 9,086 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,375,602 | 113,375,623 | GGGAACTTTCTGAGATGCACTG |
| TUP | 113,375,570 | 113,375,597 | ACAGTCAGTTGCATAGACAGAACCACCG |
| TUR | 113,375,537 | 113,375,560 | CCAGCTAACCTTTATGTCACACTC |
| TDF | 113,367,024 | 113,367,042 | GGCCTGGAAGACCCTGAAG |
| TDP | 113,366,999 | 113,367,022 | AGCGGGAAAGCATCCTGAAGCTCC |
| TDR | 113,366,973 | 113,366,991 | CAGCAGCAGGTCAGGCTAC |
PCR Primers
| Primer | Sequence |
| SU | GGCATGTGTACATACTCTTCTGG |
| SD | CTTCCACCTGCCTGTTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12099A-E5 | VGB6 | C57BL/6NTac | |
| 12099A-E6 | VGB6 | C57BL/6NTac | |
| 12099A-E11 | VGB6 | C57BL/6NTac | |
