| Gene | Fam176b |
| Chromosome | 4 |
| Deletion Start | 126,148,982 |
| Deletion End | 126,149,744 |
| Deletion Size | 763 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,149,068 | 126,149,087 | CGGAGCCCGTCCATCATAAC |
| TUP | 126,149,088 | 126,149,108 | CCAGGCAGCCCTCCACTCCTA |
| TUR | 126,149,110 | 126,149,128 | GCAAGGACAGGGTGAGCAG |
| TDF | 126,149,398 | 126,149,420 | ACGAGGACGATGAGGAAGATGAG |
| TDP | 126,149,423 | 126,149,444 | CACCATGACGAGGCTGGGTCCC |
| TDR | 126,149,505 | 126,149,524 | TCCTCTGCTGACGTGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGAGGGAGGTGGAAGG |
| SD | ATAGTATCCGCCCTGTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 127 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12101A-B3 | VGB6 | C57BL/6NTac | |
| 12101A-G2 | VGB6 | C57BL/6NTac | |
| 12101A-E11 | VGB6 | C57BL/6NTac | |
| 12101A-F2 | VGB6 | C57BL/6NTac | |
| 12101A-F11 | VGB6 | C57BL/6NTac | |
| 12101A-B4 | VGB6 | C57BL/6NTac | |
