| Gene | Mterf |
| Chromosome | 5 |
| Deletion Start | 3,891,863 |
| Deletion End | 3,890,388 |
| Deletion Size | 1,476 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,891,748 | 3,891,771 | GATTTCATTCAGGCCGTTTAGTGC |
| TUP | 3,891,715 | 3,891,743 | TGCGACAGTAAGGACAAGGAGTCTTTGGA |
| TUR | 3,891,685 | 3,891,704 | AAGTTGCTCAGCAGGTCCTC |
| TDF | 3,891,009 | 3,891,029 | GAGAGGCTGCTTTCTCTTGGA |
| TDP | 3,890,976 | 3,891,003 | CGAGGAGGAGGTACAGAGGTTTGTCCTA |
| TDR | 3,890,944 | 3,890,968 | TTCTCTGACAAGAAGACCATATTCA |
PCR Primers
| Primer | Sequence |
| SU | GGAAAGTAGACAATCTGAAGTGTG |
| SD | GAAACTACAAGGCACATTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12103A-B8 | VGB6 | C57BL/6NTac | |
| 12103A-D2 | VGB6 | C57BL/6NTac | |
| 12103B-C11 | VGB6 | C57BL/6NTac | |
| 12103B-H4 | VGB6 | C57BL/6NTac | |
