| Gene | Mrps25 |
| Chromosome | 6 |
| Deletion Start | 92,183,970 |
| Deletion End | 92,174,945 |
| Deletion Size | 9,026 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,183,051 | 92,183,069 | GCAACAGGTGCTGACCTTG |
| TUP | 92,183,025 | 92,183,050 | TGCCCATTTGATCCAGTAGGTGCATC |
| TUR | 92,182,994 | 92,183,012 | GAGCCTTGTGCCCACAAAC |
| TDF | 92,175,072 | 92,175,090 | CCTGGCTTGGTGCCCTTAC |
| TDP | 92,175,036 | 92,175,062 | TGACAGGGAAGTACAAAGCTGCTCTGA |
| TDR | 92,175,001 | 92,175,021 | CCCAGATGGCCTTGCTTAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGCAAGCCACTCTTTCC |
| SD | CCCAGCTACATTTGGCTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12105B-B6 | VGB6 | C57BL/6NTac | |
| 12105B-D6 | VGB6 | C57BL/6NTac | |
| 12105B-D8 | VGB6 | C57BL/6NTac | |
| 12105B-F10 | VGB6 | C57BL/6NTac | |
| 12105B-G12 | VGB6 | C57BL/6NTac | |
