| Gene | Ccnb1 |
| Chromosome | 13 |
| Deletion Start | 100,786,399 |
| Deletion End | 100,779,660 |
| Deletion Size | 6,740 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,786,035 | 100,786,054 | TGGGTGGAAACTTGCTTGTC |
| TUP | 100,786,009 | 100,786,033 | CGGCTGGAGGCTAGATCCTACCACC |
| TUR | 100,785,983 | 100,786,004 | GGCGGGACCTAACTAAGGAAAG |
| TDF | 100,780,057 | 100,780,076 | GCAGCACCTGGCTAAGAATG |
| TDP | 100,780,031 | 100,780,055 | AGTCATGGTGAACTGTGGCCTCACA |
| TDR | 100,779,927 | 100,779,947 | GTCCTGGCTGGCTCTAAACTC |
PCR Primers
| Primer | Sequence |
| SU | CTACCTAAGGCAGGTGGCAG |
| SD | GTTAAAGGTGGCAACCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12107A-H6 | VGB6 | C57BL/6NTac | |
| 12107A-G12 | VGB6 | C57BL/6NTac | |
| 12107A-G7 | VGB6 | C57BL/6NTac | |
