| Gene | Lipi |
| Chromosome | 16 |
| Deletion Start | 75,555,849 |
| Deletion End | 75,541,011 |
| Deletion Size | 14,839 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,555,409 | 75,555,429 | AGCACATGGTTCACAGCTCAT |
| TUP | 75,555,377 | 75,555,406 | CCACAAACACTAGAGGAATAGGTAAAGCCA |
| TUR | 75,555,317 | 75,555,339 | GAGCAAGCAAGGTGTTTAGAAGA |
| TDF | 75,541,093 | 75,541,117 | TGTATTGAAAGAAGGAACAAGGGTA |
| TDP | 75,541,071 | 75,541,090 | TCTTCAACCAGATGAATGTA |
| TDR | 75,541,049 | 75,541,068 | GGTGTTCACTCCATCTGCGT |
PCR Primers
| Primer | Sequence |
| SU | GGTGGATATGGAAATTTGGG |
| SD | CACGAACCCAGCTTATTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12108A-D8 | VGB6 | C57BL/6NTac | |
| 12108A-D11 | VGB6 | C57BL/6NTac | |
