| Gene | Rgp1 |
| Chromosome | 4 |
| Deletion Start | 43,579,397 |
| Deletion End | 43,583,119 |
| Deletion Size | 3,723 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,579,854 | 43,579,875 | AGGTTCCCATACCTTCGTTTCC |
| TUP | 43,579,878 | 43,579,900 | TTTCCACAGTGAGGCTCTGGCCT |
| TUR | 43,579,941 | 43,579,959 | TGGCAGTGCTACTCGACTC |
| TDF | 43,582,323 | 43,582,345 | TGGTTCTTTCTACCACAGGGTTC |
| TDP | 43,582,347 | 43,582,370 | TGACTCAGGTCACCTGGCTTGGTG |
| TDR | 43,582,372 | 43,582,392 | TCAGCGGGTTAAAGGGACTTG |
PCR Primers
| Primer | Sequence |
| SU | ATGTGACAAAGGAAGTCCCG |
| SD | GAAGTGCTACAGGAGGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12112A-A9 | VGB6 | C57BL/6NTac | |
| 12112A-C7 | VGB6 | C57BL/6NTac | |
