| Gene | Lilrb4 |
| Chromosome | 10 |
| Deletion Start | 51,491,030 |
| Deletion End | 51,496,245 |
| Deletion Size | 5,216 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,491,438 | 51,491,458 | CCTCCCAAAGCCCATCATCTG |
| TUP | 51,481,168 | 51,481,189 | TGAGCCAGGCTCTGTGATCGCT |
| TUR | 51,491,496 | 51,491,518 | GAACCCTGACACCAGGTAATCAC |
| TDF | 51,495,767 | 51,495,792 | CATAGGCAGAAAATACAGCAGATACG |
| TDP | 51,485,529 | 51,485,558 | TGGGAATCAGTGTGACAATAAAAGGCTGGA |
| TDR | 51,495,929 | 51,495,954 | GGTTCCATCCAGTATCTCTTTCAGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGTCCTAAAGGATGTGGC |
| SD | GTTTATTGAGGAGGATTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 188 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12116A-C7 | VGB6 | C57BL/6NTac | |
| 12116A-D3 | VGB6 | C57BL/6NTac | |
| 12116A-E12 | VGB6 | C57BL/6NTac | |
