| Gene | Lst1 |
| Chromosome | 17 |
| Deletion Start | 35,188,394 |
| Deletion End | 35,185,154 |
| Deletion Size | 3,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,188,205 | 35,188,225 | ACCCGGAAACAGTAGGGAGTG |
| TUP | 35,188,169 | 35,188,197 | TCAGGAGAGGTAACCCGGTAGGTGAATCT |
| TUR | 35,188,139 | 35,188,159 | ACCCAGACTTCAACTTCTCAC |
| TDF | 35,186,088 | 35,186,108 | GTCCTTATCCAGCGGGTGTAG |
| TDP | 35,186,062 | 35,186,084 | TGTGTTTGATTCCCAGCGCAGCA |
| TDR | 35,186,015 | 35,186,037 | CCTGCCTCTATGTGGGATTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGTCTACAGAGTGAGTTC |
| SD | AGGCCAACAATAAGTCCTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12118A-A9 | VGB6 | C57BL/6NTac | |
| 12118A-B1 | VGB6 | C57BL/6NTac | |
| 12118A-C1 | VGB6 | C57BL/6NTac | |
| 12118A-H2 | VGB6 | C57BL/6NTac | |
