| Gene | Trem1 |
| Chromosome | 17 |
| Deletion Start | 48,237,024 |
| Deletion End | 48,244,662 |
| Deletion Size | 7,643 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,237,300 | 48,237,320 | CCTCCGAATGACCCTGTTGTG |
| TUP | 48,237,322 | 48,237,344 | TCTTCCATCCTGTCCGCCTGGTG |
| TUR | 48,237,350 | 48,237,369 | TTCCTGGTCACTCACCCTTG |
| TDF | 48,244,263 | 48,244,284 | GCTGACCACAGAGGTGACATAC |
| TDP | 48,244,289 | 48,244,314 | TAGGTGAGCCTCACTCAGTCCACAGA |
| TDR | 48,244,321 | 48,244,341 | AAGGGCTAACGCTGCATCTTG |
PCR Primers
| Primer | Sequence |
| SU | TAGCATGTACTCACAAAAGCG |
| SD | GGGAAGCTCTGGAGTTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12124A-D4 | VGB6 | C57BL/6NTac | |
| 12124A-B3 | VGB6 | C57BL/6NTac | |
| 12124A-A6 | VGB6 | C57BL/6NTac | |
