| Gene | Treml2 |
| Chromosome | 17 |
| Deletion Start | 48,300,530 |
| Deletion End | 48,309,516 |
| Deletion Size | 8,987 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,301,086 | 48,301,108 | ACCGCTTACCACTTCCTGTTCTG |
| TUP | 48,301,109 | 48,301,134 | TTCTGGCTAGAGTAAGGGCTGGCAAA |
| TUR | 48,301,169 | 48,301,188 | GGGTCCCAGGTTCGTTTGTG |
| TDF | 48,308,571 | 48,308,592 | GCAGCTCCTCAGATACTCACTC |
| TDP | 48,308,594 | 48,308,622 | AGGCCAAGGGAAAGTGACATGCTAACTCC |
| TDR | 48,308,663 | 48,308,680 | GGCCTGCACACTCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTGAAGTTCTGAGGAAG |
| SD | CCTCCACGAGTTCATCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12125A-G12 | VGB6 | C57BL/6NTac | |
| 12125A-C8 | VGB6 | C57BL/6NTac | |
