| Gene | Tnfsf12 |
| Chromosome | 11 |
| Deletion Start | 69,691,839 |
| Deletion End | 69,682,924 |
| Deletion Size | 8,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,694,849 | 69,694,868 | TCTCTGGGAGGTCCTGTTTG |
| TUP | 69,694,819 | 69,694,846 | CCCTGCCCTTAATTCCTCTAGGAACTGA |
| TUR | 69,694,794 | 69,694,813 | TCCTGGCTTTCCTCTGTCTG |
| TDF | 69,687,032 | 69,687,051 | ACTTGCTGGTGAACGGTGTG |
| TDP | 69,687,005 | 69,687,027 | CCCTGCGCTGCCTGGAAGAATTC |
| TDR | 69,686,981 | 69,687,000 | AGGAGAGCTTGCTGCTGTGG |
PCR Primers
| Primer | Sequence |
| SU | TCAGAGTTCTTGGTCTGGAG |
| SD | GCTAGTGAGGTGGATAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12127A-B8 | VGB6 | C57BL/6NTac | |
| 12127A-A10 | VGB6 | C57BL/6NTac | |
| 12127A-H7 | VGB6 | C57BL/6NTac | |
