| Gene | Gas6 |
| Chromosome | 8 |
| Deletion Start | 13,494,207 |
| Deletion End | 13,465,568 |
| Deletion Size | 28,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,494,034 | 13,494,053 | CCGAGTGGGTGTAGGTTGAC |
| TUP | 13,494,007 | 13,494,030 | ACACCCTTCTTTCTGCAGCTGTGC |
| TUR | 13,493,931 | 13,493,950 | TTGGCCTCCTCGAAGACTTG |
| TDF | 13,466,317 | 13,466,334 | CTGCCCTTCGCACACTTC |
| TDP | 13,466,291 | 13,466,315 | ACCAGAAGCTAGCAAGAGCAGCCAA |
| TDR | 13,466,257 | 13,466,276 | CACCTGTGGGCCTGTGTAAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGATACAATTCTCCTTTC |
| SD | GAGTCAGAAAGAAGCATGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12128A-A11 | VGB6 | C57BL/6NTac | |
| 12128A-C2 | VGB6 | C57BL/6NTac | |
| 12128A-H3 | VGB6 | C57BL/6NTac | |
| 12128A-E3 | VGB6 | C57BL/6NTac | |
| 12128A-F2 | VGB6 | C57BL/6NTac | |
