| Gene | Igf2 |
| Chromosome | 7 |
| Deletion Start | 142,663,804 |
| Deletion End | 142,661,775 |
| Deletion Size | 2,030 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,655,118 | 142,655,135 | GCCTACCTGCCTGTGAAC |
| TUP | 142,655,090 | 142,655,117 | TGCTCTGGAAACAACTACTGCTCATGGG |
| TUR | 142,655,060 | 142,655,078 | GGCTCTCGACCAAGGACTG |
| TDF | 142,654,610 | 142,654,631 | TGGCTCACACTTCTACCACATC |
| TDP | 142,654,581 | 142,654,605 | CAGGGTGACCTAGTATCCGGCCAGG |
| TDR | 142,654,539 | 142,654,558 | GGGAAGGCTGAAGAAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGATGGGTGGTCCTCAAG |
| SD | GTCAATTGGGTTGTTTAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12130A-B8 | VGB6 | C57BL/6NTac | |
| 12130A-G11 | VGB6 | C57BL/6NTac | |
| 12130A-F8 | VGB6 | C57BL/6NTac | |
| 12130A-D8 | VGB6 | C57BL/6NTac | |
