| Gene | Ahsg |
| Chromosome | 16 |
| Deletion Start | 22,892,118 |
| Deletion End | 22,899,097 |
| Deletion Size | 6,980 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,892,695 | 22,892,719 | TAAATCCTCCCTTCATTCATTCCAG |
| TUP | 22,892,723 | 22,892,750 | TGCCTGATAGCCATGTTCTCCTTAGACA |
| TUR | 22,892,771 | 22,892,791 | GGCGGTTTCATAGTTTCAGTC |
| TDF | 22,898,574 | 22,898,596 | TGTGGAAATCTACTGGGACTGAG |
| TDP | 22,898,598 | 22,898,621 | TAATGCAGGTGAGACTGGCGCTCC |
| TDR | 22,898,647 | 22,898,671 | AAGGAGAATGGGTAAGATGCTAAGG |
PCR Primers
| Primer | Sequence |
| SU | AGCAGCATTGACTTTGGCAG |
| SD | TTCAGAGTCGTGGTTGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12131A-H5 | VGB6 | C57BL/6NTac | |
| 12131D-G1 | VGB6 | C57BL/6NTac | |
