| Gene | Enho |
| Chromosome | 4 |
| Deletion Start | 41,638,663 |
| Deletion End | 41,636,598 |
| Deletion Size | 2,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,640,918 | 41,640,936 | GGGCCATTCTTCAGGTGTC |
| TUP | 41,640,893 | 41,640,917 | TCAAGTTCCCTCCAGCAAGCCATGC |
| TUR | 41,640,872 | 41,640,891 | CAGTGTTGGTCACAGGGTTG |
| TDF | 41,640,481 | 41,640,500 | CTCCTTCTCTCCTGCACCAG |
| TDP | 41,640,457 | 41,640,479 | ATGGTCGTGTCCCACCCCAGAGC |
| TDR | 41,640,434 | 41,640,451 | CAAGTTCCCGCGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | TCTTCTGGGCTCTTTATCAC |
| SD | GTCTGAACGTACATCTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12133E-D2 | VGB6 | C57BL/6NTac | |
| 12133F-F9 | VGB6 | C57BL/6NTac | |
