| Gene | Fgfr1 |
| Chromosome | 8 |
| Deletion Start | 25,555,513 |
| Deletion End | 25,573,922 |
| Deletion Size | 18,410 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,555,709 | 25,555,727 | TCTACGCTTGCGTGACCAG |
| TUP | 25,555,733 | 25,555,758 | CCTCTGGCAGCGATACCACCTACTTC |
| TUR | 25,555,783 | 25,555,802 | GGATAGGGCAGAGGGCATTG |
| TDF | 25,573,324 | 25,573,341 | GGCAAGCTGGGTTGGATG |
| TDP | 25,573,347 | 25,573,370 | ACAGCTTCCTCAGGTGATGGTGGC |
| TDR | 25,573,406 | 25,573,426 | CACCATGCTCCTGCTTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTGATAGAGCCATCTGAAC |
| SD | TGCACACCTCCAAGCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12134A-H1 | VGB6 | C57BL/6NTac | |
| 12134A-F2 | VGB6 | C57BL/6NTac | |
| 12134A-B4 | VGB6 | C57BL/6NTac | |
