| Gene | Nampt |
| Chromosome | 12 |
| Deletion Start | 32,820,573 |
| Deletion End | 32,850,549 |
| Deletion Size | 29,977 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,821,091 | 32,821,109 | GGTTGCAGAGGCCAGTGTG |
| TUP | 32,821,111 | 32,821,137 | TCTGGCTCTCCAGGATCAACTACGTGA |
| TUR | 32,821,155 | 32,821,179 | GTTCCTTTAACTCTTGGTGTTTCAC |
| TDF | 32,849,635 | 32,849,654 | CCATCGAAGACGCAGCAGAG |
| TDP | 32,849,656 | 32,849,684 | CCTGAGACTAAGCCAAAGGAAACTTGCCA |
| TDR | 32,849,703 | 32,849,723 | GACTGGTCACTAGGACACTGG |
PCR Primers
| Primer | Sequence |
| SU | AAAGAGACTCCCGGCAGGA |
| SD | AACACCAGATTGGATTACTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12136A-F2 | VGB6 | C57BL/6NTac | |
| 12136A-H11 | VGB6 | C57BL/6NTac | |
