| Gene | Rbp4 |
| Chromosome | 19 |
| Deletion Start | 38,125,005 |
| Deletion End | 38,117,249 |
| Deletion Size | 7,757 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,124,543 | 38,124,561 | GGTGAGCAGCTTCCGAGTC |
| TUP | 38,124,512 | 38,124,541 | AGGAGAACTTCGACAAGGCTCGTGTAGGTA |
| TUR | 38,124,464 | 38,124,482 | AGTCTGGGCTCCACCTCTG |
| TDF | 38,117,041 | 38,117,061 | GAGCATCAACTGTCAGGGAAC |
| TDP | 38,117,011 | 38,117,039 | TGTGAAGTAAGCCATACCGATGCCTATGA |
| TDR | 38,116,986 | 38,117,005 | GGGCAGCCATAGAGCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CGGTTACGAAAGCGCGACC |
| SD | AACTCATAGGAAGATGGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12137B-G5 | VGB6 | C57BL/6NTac | |
| 12137A-C5 | VGB6 | C57BL/6NTac | |
