| Gene | Serpina12 |
| Chromosome | 12 |
| Deletion Start | 104,042,368 |
| Deletion End | 104,034,844 |
| Deletion Size | 7,525 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,038,206 | 104,038,228 | TGCTCGACACAACATGGAATTTG |
| TUP | 104,038,180 | 104,038,203 | TTCAAGCTACTGCAGAGGCTGGCC |
| TUR | 104,038,126 | 104,038,145 | GGCAGTGGAGATGCTCAGTG |
| TDF | 104,030,992 | 104,031,011 | GCATTCCCTGTTGGCTTCAG |
| TDP | 104,030,965 | 104,030,988 | CCACCCATTTCCAAGGGTTGCACC |
| TDR | 104,030,932 | 104,030,951 | CTGGTGGGTGGCATTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGATGACAAAGATTCGATGC |
| SD | GTGGCTTTCCTAGCAATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12138A-G2 | VGB6 | C57BL/6NTac | |
| 12138A-B3 | VGB6 | C57BL/6NTac | |
