| Gene | Slc2a9 |
| Chromosome | 5 |
| Deletion Start | 38,398,320 |
| Deletion End | 38,368,064 |
| Deletion Size | 30,257 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,482,704 | 38,482,724 | CATCCCTGCTCTACCTGTTTC |
| TUP | 38,482,676 | 38,482,703 | TGCTTCCAGGAGGTCTTCAGTAGAAGGT |
| TUR | 38,482,628 | 38,482,651 | TTGCCTGAAGGAATGATCTTTGTC |
| TDF | 38,453,190 | 38,453,208 | GACCCGGATACCCTGACTC |
| TDP | 38,453,166 | 38,453,189 | TGCTCTGGTCTGTGACTGTGTCCA |
| TDR | 38,453,130 | 38,453,148 | CCATCAGCGTTCCCACCAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAGTGCTCTACCACTGAG |
| SD | CATGCAGTGAAGTCTTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12141A-D2 | VGB6 | C57BL/6NTac | |
| 12141A-D4 | VGB6 | C57BL/6NTac | |
| 12141A-D10 | VGB6 | C57BL/6NTac | |
| 12141A-D11 | VGB6 | C57BL/6NTac | |
| 12141A-G5 | VGB6 | C57BL/6NTac | |
