| Gene | Azgp1 |
| Chromosome | 5 |
| Deletion Start | 137,981,563 |
| Deletion End | 137,989,895 |
| Deletion Size | 8,333 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,981,792 | 137,981,810 | TGGCTCGGTTGAGAGGATG |
| TUP | 137,981,812 | 137,981,836 | TTGTTGTGGATGCAGGAAGCCTTCA |
| TUR | 137,981,844 | 137,981,866 | ACTTGGTTTATGTGGTGCTTAGG |
| TDF | 137,989,584 | 137,989,604 | TGTGATCCCAGGAGGAAACAG |
| TDP | 137,989,610 | 137,989,636 | TCAAATGCCTGGCCTATGGCTTCTACC |
| TDR | 137,989,637 | 137,989,660 | TCCAGTGCAGACTAATTCTTTGTG |
PCR Primers
| Primer | Sequence |
| SU | TATACAGACCTCCACTCCAC |
| SD | TCTGTGCTGTTCTAGGCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12142A-G2 | VGB6 | C57BL/6NTac | |
| 12142A-F10 | VGB6 | C57BL/6NTac | |
| 12142A-A7 | VGB6 | C57BL/6NTac | |
