| Gene | Car9 |
| Chromosome | 4 |
| Deletion Start | 43,507,059 |
| Deletion End | 43,513,117 |
| Deletion Size | 6,059 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,507,302 | 43,507,322 | TCTGAAGACAGGATGGAGGAG |
| TUP | 43,507,324 | 43,507,352 | CCCTTGGGTTAGAGGATCTATCGACTCCC |
| TUR | 43,507,367 | 43,507,385 | CCCGTGGGAACCTTGACTG |
| TDF | 43,512,864 | 43,512,887 | CCTTGGTGTTTGGCCTTCTCTTTG |
| TDP | 43,512,889 | 43,512,912 | TGTCACCAGCATCGCCTTCCTCCT |
| TDR | 43,512,919 | 43,512,939 | AGATACCTGTGCTGCCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTGGTGATCCTGATAACC |
| SD | CAAGAATTGAGCCCAATCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12143A-G9 | VGB6 | C57BL/6NTac | |
| 12143D-A12 | VGB6 | C57BL/6NTac | |
| 12143E-F10 | VGB6 | C57BL/6NTac | |
| 12143E-H3 | VGB6 | C57BL/6NTac | |
| 12143D-D9 | VGB6 | C57BL/6NTac | |
