| Gene | Dll1 |
| Chromosome | 17 |
| Deletion Start | 15,374,977 |
| Deletion End | 15,367,708 |
| Deletion Size | 7,270 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,374,565 | 15,374,586 | CAAGGGCCACTGATAAGGGTTG |
| TUP | 15,374,536 | 15,374,561 | AATCCCACAAAGACACGCCTGAGGTC |
| TUR | 15,374,508 | 15,374,528 | TGCCAGTCAGTTCCCAGTAAG |
| TDF | 15,368,099 | 15,368,117 | CTTGTCGTGGACGGGAGTG |
| TDP | 15,368,072 | 15,368,095 | TGGAGAGGGTCCATTTCTGCCTGC |
| TDR | 15,368,015 | 15,368,036 | CACATCGCTTCCATCTTACACC |
PCR Primers
| Primer | Sequence |
| SU | GACGAAGAGGAAACCGAAAG |
| SD | GTAATCCAAGATAGACGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12145A-A3 | VGB6 | C57BL/6NTac | |
| 12145A-A7 | VGB6 | C57BL/6NTac | |
| 12145A-G1 | VGB6 | C57BL/6NTac | |
| 12145A-F2 | VGB6 | C57BL/6NTac | |
| 12145A-A4 | VGB6 | C57BL/6NTac | |
| 12145A-B3 | VGB6 | C57BL/6NTac | |
