| Gene | Edn1 |
| Chromosome | 13 |
| Deletion Start | 42,301,898 |
| Deletion End | 42,306,893 |
| Deletion Size | 4,996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,302,769 | 42,302,787 | GCCAGAGGGAGGTGTCAAG |
| TUP | 42,302,794 | 42,302,821 | AGCAAGCTGTGCTGCCCAAAGATTCTGA |
| TUR | 42,302,841 | 42,302,859 | TCAGGGAACCCAGGGAAAG |
| TDF | 42,306,783 | 42,306,804 | GGCCATCAGCAATAGCATCAAG |
| TDP | 42,306,812 | 42,306,838 | TTCGTGTTGCAAAGTTGAAAGCTGAGC |
| TDR | 42,306,852 | 42,306,871 | CTCGGTTGTGCGTCAACTTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGGAATAAAGTCAGAGC |
| SD | TCTCTGAGAAGTCCTCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12146A-C11 | VGB6 | C57BL/6NTac | |
| 12146A-D4 | VGB6 | C57BL/6NTac | |
| 12146A-F5 | VGB6 | C57BL/6NTac | |
| 12146A-G6 | VGB6 | C57BL/6NTac | |
