| Gene | Adm |
| Chromosome | 7 |
| Deletion Start | 110,628,282 |
| Deletion End | 110,629,175 |
| Deletion Size | 894 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,628,545 | 110,628,562 | GTCGTGGGAAGAGGGAAC |
| TUP | 110,628,565 | 110,628,589 | CAAGCATCCAGCAGCTACCCTACGG |
| TUR | 110,628,606 | 110,628,628 | CAAGAGTCTGGGTAGGAACTGTC |
| TDF | 110,628,872 | 110,628,893 | GAAGCCCACATTCGTGTCAAAC |
| TDP | 110,628,895 | 110,628,918 | CTACCGCCAGAGCATGAACCAGGG |
| TDR | 110,628,928 | 110,628,946 | CCCGAAGCGGCATCCATTG |
PCR Primers
| Primer | Sequence |
| SU | GCACTTCTCAATGGCTTCCT |
| SD | ATGAGGCTTATGCCCCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12150A-C5 | VGB6 | C57BL/6NTac | |
| 12150A-E3 | VGB6 | C57BL/6NTac | |
| 12150A-F10 | VGB6 | C57BL/6NTac | |
| 12150A-D2 | VGB6 | C57BL/6NTac | |
