| Gene | Nrp2 |
| Chromosome | 1 |
| Deletion Start | 62,704,208 |
| Deletion End | 62,738,465 |
| Deletion Size | 34,258 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,704,785 | 62,704,806 | TCTTTGCAGACTGGGTGGATAC |
| TUP | 62,704,809 | 62,704,833 | CTCAGAACCAGAGGGCTGAAGCTGG |
| TUR | 62,704,856 | 62,704,875 | CACGGGAAAGCTCCCATCTC |
| TDF | 62,738,199 | 62,738,216 | TAGGGCAAGCCCAAGGAG |
| TDP | 62,738,220 | 62,738,244 | AAAGGTCCCTTTCCAGGCCTTCAGA |
| TDR | 62,738,245 | 62,738,267 | GAGACCAGATGAGGAACGAACAG |
PCR Primers
| Primer | Sequence |
| SU | TTGAAGAGGAAACCGGTCTC |
| SD | GGCTGGTGCTTCATCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12151A-H12 | VGB6 | C57BL/6NTac | |
