| Gene | Csf3 |
| Chromosome | 11 |
| Deletion Start | 98,701,383 |
| Deletion End | 98,702,964 |
| Deletion Size | 1,582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,701,936 | 98,701,957 | AGGAAGGTAGGGAGCTGAGAAG |
| TUP | 98,701,958 | 98,701,981 | CCAGGAGGACCTGAGCTGGACAAA |
| TUR | 98,702,013 | 98,702,034 | GGTGGCACACTGTAGATGGAAG |
| TDF | 98,702,353 | 98,702,374 | ACTGCCTAACCCTGCTATCTTC |
| TDP | 98,702,376 | 98,702,398 | CCAGACACAGTGCCTAAGCCAGC |
| TDR | 98,702,416 | 98,702,435 | TGCAGGAGACCTTGGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | AACTCGGATCCAGATCCAAC |
| SD | CAGCAGGAATCAATACTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12153A-B7 | VGB6 | C57BL/6NTac | |
| 12153A-C6 | VGB6 | C57BL/6NTac | |
| 12153A-D6 | VGB6 | C57BL/6NTac | |
| 12153A-G3 | VGB6 | C57BL/6NTac | |
| 12153A-G9 | VGB6 | C57BL/6NTac | |
| 12153A-F6 | VGB6 | C57BL/6NTac | |
