| Gene | Prokr1 |
| Chromosome | 6 |
| Deletion Start | 87,588,112 |
| Deletion End | 87,580,450 |
| Deletion Size | 7,663 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,588,436 | 87,588,453 | TGCGCCTCTGTCAACTAC |
| TUP | 87,588,412 | 87,588,434 | TGCGTACCGTCTCCCTCTACGTC |
| TUR | 87,588,371 | 87,588,391 | ACTCACCTGTCAATGGCAATG |
| TDF | 87,581,921 | 87,581,945 | GGGTTGACTCCTCTTCTGATCTTAC |
| TDP | 87,581,895 | 87,581,919 | CAGGTATCTGGCCATTGTGCACCCG |
| TDR | 87,581,862 | 87,581,880 | GGCGGCTGTTTGACACTTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGTCTGCATAGGTTTCAG |
| SD | CTTTTCCAGACACTCCAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12154A-E1 | VGB6 | C57BL/6NTac | |
| 12154A-F2 | VGB6 | C57BL/6NTac | |
| 12154A-E11 | VGB6 | C57BL/6NTac | |
| 12154A-B10 | VGB6 | C57BL/6NTac | |
