| Gene | S100a8 |
| Chromosome | 3 |
| Deletion Start | 90,669,545 |
| Deletion End | 90,670,026 |
| Deletion Size | 482 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,669,563 | 90,669,584 | GCCTTGAGCAACCTCATTGATG |
| TUP | 90,669,599 | 90,669,628 | TCCAATATACAAGGAAATCACCATGCCCTC |
| TUR | 90,669,671 | 90,669,692 | ACCTCCTCACCTGCACAAACTG |
| TDF | 90,669,902 | 90,669,922 | GAGGAGTTCCTTGCGATGGTG |
| TDP | 90,669,930 | 90,669,953 | TGGGTGTGGCATCTCACAAAGACA |
| TDR | 90,669,964 | 90,669,982 | AGGCCAGAAGCTCTGCTAC |
PCR Primers
| Primer | Sequence |
| SU | TAGAGCCTTCTAGCAGTGTC |
| SD | CTACCAAGTCTAAGTTACAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12157A-B4 | VGB6 | C57BL/6NTac | |
| 12157A-G1 | VGB6 | C57BL/6NTac | |
| 12157A-H2 | VGB6 | C57BL/6NTac | |
