| Gene | Itga9 |
| Chromosome | 9 |
| Deletion Start | 118,606,931 |
| Deletion End | 118,636,616 |
| Deletion Size | 29,686 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,607,774 | 118,607,794 | GCGCTTAGAGAAGGCGGATAG |
| TUP | 118,607,796 | 118,607,822 | CCACTTCCTTGGAATGAACGAGCCACA |
| TUR | 118,607,828 | 118,607,848 | CTTGAATAGGTGGGCGTGGTG |
| TDF | 118,636,196 | 118,636,217 | CCAAACCTAATGCTCTGCTCAC |
| TDP | 118,636,220 | 118,636,247 | CCTTTGCACCAAATGCTTCTCTTTCCGG |
| TDR | 118,636,271 | 118,636,288 | GGACAACACCCTCCACAC |
PCR Primers
| Primer | Sequence |
| SU | ATGCAGTTCACCGAGGTCC |
| SD | AACACAGTAGTCTCTGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12163D-G11 | VGB6 | C57BL/6NTac | |
| 12163E-C1 | VGB6 | C57BL/6NTac | |
| 12163E-G3 | VGB6 | C57BL/6NTac | |
| 12163E-G4 | VGB6 | C57BL/6NTac | |
