| Gene | Wisp1 |
| Chromosome | 15 |
| Deletion Start | 66,891,569 |
| Deletion End | 66,919,550 |
| Deletion Size | 27,982 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,891,865 | 66,891,883 | GGATCTGCACCCTGGACTG |
| TUP | 66,891,885 | 66,891,910 | CCCTGTGATCTTGGGTGGGTCACTCA |
| TUR | 66,891,942 | 66,891,964 | TGGTAGGCTCTGGATGTTCTTAG |
| TDF | 66,919,220 | 66,919,238 | TGTCAGCACACGCACCTAC |
| TDP | 66,919,239 | 66,919,264 | CGACCCAAGTACTGCGGAGTCTGTAC |
| TDR | 66,919,289 | 66,919,311 | CCACACTGATGGTCTTGGACTTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGGAGTCTGGGACTTTCC |
| SD | AGGAAGGACTTCATGATACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12164A-E5 | VGB6 | C57BL/6NTac | |
| 12164A-G1 | VGB6 | C57BL/6NTac | |
