12165 Cd97 Status: ES cell colonies screened / QC no positives
| Gene | Cd97 |
| Chromosome | 8 |
| Deletion Start | 83,740,672 |
| Deletion End | 83,730,242 |
| Deletion Size | 10,431 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,733,804 | 83,733,825 | AGGCTGACCTTCCATTCCACTC |
| TUP | 83,733,774 | 83,733,800 | CACTGGTATTGTTCAAGTCCCGGGTCC |
| TUR | 83,733,742 | 83,733,760 | GGTCTGCTCTGCTCCTCTC |
| TDF | 83,724,066 | 83,724,086 | TGTTCACAGGTGAGGGAAGAG |
| TDP | 83,724,040 | 83,724,063 | CTGGAAATGGGCCTGCATGGTCAC |
| TDR | 83,723,998 | 83,724,018 | GTGCCTGTTGTGGAAGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGTCTGTCTCTTTGTATG |
| SD | TCTTCCCTTTGTCCTAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
